D(-)Lactic Acid Formation and D(-)Lactate Dehydrogenase in Octopus Spermatozoa

A. W. Martin, R. Jones, T. Mann

Abstract

Spermatozoa of Octopus dofleini martini produce D(-)lactic acid in the course of their metabolism, and they also possess a highly active D(-)-lactate dehydrogenase. This distinguishes the octopus spermatozoa from mammalian spermatozoa which form L(+)lactic acid and have a L(+)-lactate dehydrogenase. The D(-)lactate dehydrogenase has been extracted from the octopus testis. It was shown to be a D(-)lactate:NAD oxidoreductase with electrophoretic behaviour different from the D(-)-lactate dehydrogenases present in either the horseshoe crab muscles or in Lactobacillus leichmannii.