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Atmospheric oxygen level and the evolution of insect body size

Jon F. Harrison, Alexander Kaiser, John M. VandenBrooks
Published 10 March 2010.DOI: 10.1098/rspb.2010.0001
Jon F. Harrison
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Alexander Kaiser
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John M. VandenBrooks
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Abstract

Insects are small relative to vertebrates, possibly owing to limitations or costs associated with their blind-ended tracheal respiratory system. The giant insects of the late Palaeozoic occurred when atmospheric PO2 (aPO2) was hyperoxic, supporting a role for oxygen in the evolution of insect body size. The paucity of the insect fossil record and the complex interactions between atmospheric oxygen level, organisms and their communities makes it impossible to definitively accept or reject the historical oxygen-size link, and multiple alternative hypotheses exist. However, a variety of recent empirical findings support a link between oxygen and insect size, including: (i) most insects develop smaller body sizes in hypoxia, and some develop and evolve larger sizes in hyperoxia; (ii) insects developmentally and evolutionarily reduce their proportional investment in the tracheal system when living in higher aPO2, suggesting that there are significant costs associated with tracheal system structure and function; and (iii) larger insects invest more of their body in the tracheal system, potentially leading to greater effects of aPO2 on larger insects. Together, these provide a wealth of plausible mechanisms by which tracheal oxygen delivery may be centrally involved in setting the relatively small size of insects and for hyperoxia-enabled Palaeozoic gigantism.

1. Introduction

If the insects had hit on a plan for driving air through their tissues instead of letting it soak in, they might have become as large as lobsters, though other considerations would have prevented them from becoming as large as man. (Haldane 1926)More than 300 Ma, giant insects up to 10-fold larger than those in similar groups alive today roamed the earth (Shear & Kukalova-Peck 1990; Grimaldi & Engel 2005). It has been proposed that atmospheric hyperoxia (defined here as atmospheric oxygen partial pressures (aPO2) greater than the current 21 kPa) in the Palaeozoic was the major factor allowing the evolution of giant insects and other animals, with subsequent hypoxia (defined here as aPO2 below present day) being responsible for their disappearance (Graham et al. 1995; Dudley 1998; Berner 2006b; Ward 2006). While a number of evolutionary events have been tied to changes in atmospheric oxygen level (Berner et al. 2007), the hypothesis that variation of aPO2 is responsible for historical changes in the body size of insects has recently been challenged owing to uncertainties in physiological mechanisms and in patterns of oxygen and body size variation through time (Butterfield 2009).

2. Geological and palaeontological evidence for changes in atmospheric oxygen level as a cause of historical insect gigantism

Recent atmospheric modelling efforts suggest that oxygen levels have varied dramatically from the initial evolution of insects in the late Silurian (Grimaldi & Engel 2005) to the present day. These models are based on a number of approaches including the balance of sedimentary rock abundance (Berner & Canfield 1989), carbon and sulphur isotopic changes (Berner 2005, 2006a,b), and incorporating feedbacks from atmospheric oxygen levels and fire on biogeochemical cycling (Bergman et al. 2004). Although some aspects of the pattern of aPO2 during the Phanerozoic have been controversial (e.g. Triassic hypoxia, Cretaceous hyperoxia and Tertiary oxygen stability), all the models agree on one major point—a period of hyperoxia spanning the Carboniferous and Permian reaching a maximum of 27–35 kPa (figure 1). The large-scale variation in oxygen modelling results during the Triassic to Tertiary (figure 1) should act as a general warning of associating oxygen variation in time periods other than the Permo-Carboniferous to evolutionary events.

Figure 1.
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Figure 1.

Various patterns of modelled aPO2 over the Phanerozoic. Solid line (Berner & Canfield 1989), dotted line (Berner 2006b), dashed line (Bergman et al. 2004).

In 1995, Graham et al. pointed out that the high oxygen spike in the Permo-Carboniferous was coincident with the rise of giant Palaeozoic animals in diverse taxa, including insects, millipedes, chelicerates and amphibians. Giant dragonflies of the extinct order Protodonata (Meganeura) had wingspans exceeding 70 cm in length, with over five times the length and twice the thoracic width of the largest extant dragonflies (May 1982; Shear & Kukalova-Peck 1990; Carpenter 1992). Among insects, gigantism in the Permo-Carboniferous has also been reported for Ephemeroptera, Diplura, Thysanura and the extinct order Paleodictyoptera (Briggs 1985; Kukalova-Peck 1985). Arthropleura, a group related to modern day millipedes, reached upwards of 2 m in length, almost six times the size of any extant millipede.

However, a critical analysis must conclude that the palaeontological evidence for a link between insect size and atmospheric oxygen levels is, at best, weakly correlational. The evidence for insect gigantism in the Permo-Carboniferous is based on a few documented large fossils, raising the possibility that this is a sampling artefact. Extant species represent a small proportion of all species that have ever occurred, and so it is not surprising that larger species than today's existed in the past; also larger species tend to be better-preserved (Grimaldi & Engel 2005). The loss of giant insects in the late Permian coincided with a major loss of insect diversity (Labandera & Sepkoski 1993), and it is not clear that larger species were differentially affected. While there are reports of gigantic Ephemeroptera in the late Cretaceous (Carpenter 1992); since some models predict hyperoxia and others hypoxia during that time (figure 1), it is presently unclear whether this observation supports a link between hyperoxia and insect gigantism (Dudley 2000). The majority of Permo-Carboniferous insects were not giants; plots of the size distributions of fossil insects from the Wellington formation of the Lower Permian demonstrate that most insects from this time period were moderately sized, with a few large species (Beckemeyer & Hall 2007). Similarly, extant insects often exhibit right-skewed size distributions with mostly moderately sized species and a few very large ones (Allen et al. 2006; Chown & Gaston 2010). While the largest insects were clearly bigger in the Permo-Carboniferous than in any other time period, we do not know whether average insect size was larger. Because laboratory studies suggest strong effects of oxygen on both average and maximal body size (see below), statistical demonstration of positive correlations between modelled atmospheric oxygen level and average or maximal fossil size would be extremely useful for building the case for a link between oxygen and insect size.

The palaeontological data supporting effects of aPO2 on body size is somewhat stronger for other taxonomic groups. Falkowski et al. (2005) showed that mammalian body size over the last 65 Myr gradually increased with the aPO2 predicted by the Berner et al. models. Similarly, the average body and head size of Reptilomorph fossils ranging from the Upper Carboniferous to the Upper Permian was strongly positively correlated with modelled aPO2 (VandenBrooks 2007). Correlations between aPO2 and body size in multiple, independently evolved animal lineages certainly support the hypotheses that aPO2 affects body size through evolutionary time.

3. Mechanisms for oxygen effects on insect body size

Perhaps the most relevant evidence would be long-term experiments on the effects of abnormally high or low oxygen partial pressures on the living representatives of the many groups which exist today. (McAlester 1970)Body size is affected by a wide range of proximate and ultimate factors. Proximately, adult body size is the product of growth rate and development time. Genes affect growth rate by influencing capacities to consume, process and assimilate food, and the hormonally regulated transitions to a sexually mature state that determine development time. These intrinsic factors are modulated by environmental factors such as food availability, food quality and temperature. A variety of hormones and signalling cascades regulate growth rate and development time, including insulin, target of rapamycin, ecdysone and juvenile hormone (Nijhout 2006; Mirth & Riddiford 2007).

Many ultimate factors affect the evolution of animal size (Allen et al. 2006; Chown & Gaston 2010). In a variety of insects, reproductive success, including fecundity in females and competitive ability of males, increases with individual size, owing to positive correlations between adult size and egg size and number, foraging ability, fighting ability and capacities to survive resource dearths (Brown & Mauer 1986). Nonetheless, most species have relatively small sizes, as smaller animals tend to be more viable, requiring less space, development time and nutrients to reach adulthood (Blanckenhorn 2000). Ecological factors such as temperature, nutrient availability, community interactions (competition and predator–prey), the match between the size of animals and their food and season length also affect body size distributions (Allen et al. 2006; Chown & Gaston 2010). Taxon-specific physiological or biomechanical constraints can influence body size distributions; certainly, terrestrial arthropods in general, and insects in particular, are usually smaller than vertebrates, a pattern that remains to be definitively explained.

Multiple mechanisms can be suggested for how changing aPO2 can affect insect body size. At the individual level (figure 2a), positive correlations between PO2, metabolic rates and performance (e.g. locomotion, feeding) could increase food intake, growth and survival. Positive correlations between aPO2 and oxidative stress could reduce growth and survival. Insects alter the resistance of their tracheal system in compensatory responses to aPO2 (e.g. greater ventilation and spiracular opening at lower aPO2), affecting respiratory water loss rates, growth and survival. Changing internal PO2 will also affect developmental processes mediated by signalling pathways such as insulin and hypoxia inducible factor (HIF) that influence protein synthesis, cell size and number, and body size (Gorr et al. 2006; Dekanty et al. 2007). HIF-signalling mediates compensatory changes in tracheal diameters and branching in response to aPO2, dampening effects of aPO2 on internal PO2 and altering the allocation of body materials, energy and space between the respiratory and non-respiratory system. Because HIF and hypoxic regulation of growth are widespread in the animal kingdom (Gorr et al. 2006), it is reasonable to suggest that similar responses would have been present in Palaeozoic insects.

Figure 2.
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Figure 2.

Plausible mechanisms by which atmospheric oxygen partial pressure (aPO2) might affect the evolution of insect size. Straight arrows indicate positive correlations, dashed negative, and both arrows together indicate nonlinear effects. Circles next to a trait indicate empirical support for an effect of hypoxia (open circles), or both hypoxia and hyperoxia (semi-filled circles) in Drosophila melanogaster. At the individual level (a), changes in aPO2 result in changes in internal PO2 that are damped relative to the environment owing to changes in the resistance of the tracheal system. Changing tracheal system resistance affects water loss rates, altering fitness in some environments. Internal PO2 causes direct, acute effects (left box), altering levels of oxidative stress and affecting performance variables such as flight or feeding. Changes in performance can directly affect fitness; for example, changing agility might improve foraging or the ability to escape predators. Internal PO2 also causes developmental effects (right box) via oxygen-sensitive signalling systems that control development time, cell size and number, that then affect body size and fitness. Developmental processes allow compensatory changes in respiratory structures; altering relative investment in non-respiratory structures such as muscles and ovaries that may affect fitness. The within-individual effects may influence average or maximal size of all or just the largest species. Some of the multiple possibilities include (b) alterations in abundance that affect the range but not mean size, (c) evolutionary shifts in mean size that increase over generations owing to size*aPO2 interactions or (d) effects only on the largest individuals or species, such as relief of a constraint on maximal size. Over multiple generations, individuals within the new population cycle through the changed aPO2, causing enhanced evolution of body size.

Changing oxygen level over multiple generations may affect just the maximal size of the largest species, but could also affect the average size of most species. As one example, if higher aPO2 generally improves viability and fecundity for individuals, but the body size that optimizes fitness is unaffected, an increase in abundance and range of body sizes is probable, increasing maximal without affecting mean size (figure 2b). Alternatively, intrinsically larger animals may benefit more from higher aPO2, leading to evolutionary changes in size. For example, individuals may vary in their genetically determined body and jaw sizes, with larger size allowing greater feeding, growth, size and fecundity under good environmental conditions, but reducing survival under poor conditions. If higher aPO2 improves environmental conditions (e.g. by enabling more foraging or reducing costs of respiration), individuals with larger bodies and jaws may benefit more. A significant size*oxygen interaction favouring intrinsically larger animals will tend to shift the size distribution, increasing mean and maximal population body size (figure 2c). Finally, aPO2 might only affect the largest individuals within a species or the largest species. For example, higher aPO2 might relieve some upper constraint on body size, leading to increases in size of the very largest individuals (figure 2d).

The remainder of this section will examine the empirical data related to the various possible pathways in figure 2. We begin by examining the capacity of insects to dampen the effect of aPO2 by behavioural, physiological and morphological compensation. Next we review the evidence that aPO2 has acute effects on insect performance that could affect size or fitness. We continue by considering developmental responses to aPO2 that affect body size distributions. Finally, we examine the existing data that aPO2 affects insect size evolution. Empirical support for specific mechanisms (arrows in figure 2) helps build support for the hypothesis that changing aPO2 affects insect size evolution, but the multiple plausible pathways in series and parallel (figure 2) make it challenging to determine cause and effect.

(a) Acute respiratory compensation to varying aPO2

Most animals, including insects, exhibit compensatory changes in respiratory function in response to changing oxygen levels. In insects, these changes include adjusting the degree and duration of opening of spiracular valves, alteration of fluid levels in tracheoles, and changes in ventilation (Harrison et al. 2006). In all cases, these responses are compensatory, with tracheal system resistance increasing at higher aPO2, dampening changes in internal PO2. The positive correlation between aPO2 and tracheal system resistance leads to a negative correlation between aPO2 and respiratory water loss (Lighton et al. 2004).

(b) Acute effects of aPO2 on insect performance

Critical PO2 (the aPO2 below which a process is oxygen-limited) values are highest for highly aerobic behaviours such as flight (Harrison et al. 2006). Hyperoxia increases flight metabolism and performance in one dragonfly species (Harrison & Lighton 1998), but had no effect relative to normoxia in honey bees (Joos et al. 1997) or grasshoppers (Rascón & Harrison 2005). Critical PO2 values for feeding rates are 5 kPa in Manduca sexta caterpillars (Greenlee & Harrison 2005) and 10 kPa in Drosophila melanogaster larvae (Frazier 2007). For oxygen delivery in resting insects, critical PO2 values average 6 kPa (Harrison et al. 2006). Thus the empirical data available suggest that moderate hypoxia probably depresses peak aerobic performance of many insects, while moderate hyperoxia generally has few acute effects. Similar results have been found for most air-breathing vertebrates, with only the most aerobic mammals (e.g. racehorses) showing a stimulation of metabolism and performance by hyperoxia (Jones 1994).

If larger insects have higher critical PO2 values, this could provide a mechanism driving evolution of larger size in hyperoxia (figure 2c,d). However, three ontogenic studies on resting grasshoppers (Greenlee & Harrison 2004a), jumping grasshoppers (Kirkton et al. 2005) and feeding caterpillars (Greenlee & Harrison 2005) have found no evidence for an increase in the critical PO2 values with size across developmental instars, suggesting that the capacity of the tracheal respiratory delivery system is at least matched to tissue needs as insects grow (but see Kirkton et al. (2005) for some contrary evidence). In fact the highest critical PO2 values may occur in the smallest, earliest developmental stages in grasshoppers (Greenlee & Harrison 2004a). Similarly, the greatest oxygen sensitivity measured for M. sexta occurs in the eggs, whose metabolic and development rates are oxygen-limited at temperatures of 27°C and higher, and improved by hyperoxia (Woods & Hill 2004). Interestingly, critical PO2 values rise dramatically late within each instar, as metabolic rates increase with tissue growth while tracheal systems may be compressed before moulting (Greenlee & Harrison 2004b, 2005). Together these studies provide evidence that critical PO2 does not generally increase with size, but it would be useful to study this question during specific behaviours or life stages when oxygen is most limiting.

(c) Developmental plasticity in response to changes in aPO2

Recent studies indicate that developmental plasticity causes most insects to be smaller when reared in moderate to severe hypoxia, and some species to grow larger when reared in hyperoxia (Harrison et al. 2009). The developmental responses of growth and size to atmospheric oxygen levels affect both the mean and maximal size of populations, and can be caused by both changes in growth rates and development time. In addition, compensatory morphological changes in the tracheal respiratory system to variation in aPO2 are striking.

(i) Responses to hypoxia

Hypoxia has strong effects on growth and size of insects (developmental plasticity), reducing body size in eight of nine examined insect species: in the fruitfly D. melanogaster, the mealworm beetles Zophobas morio and Tenebrio molitor, the scarabaeid beetle Cotinus texana, the cockroach Blatella germanica, the tobacco hornworm moth M. sexta and the mayfly Hexagenia limbata (Winter et al. 1996; Harrison et al. 2009). The exception was the grasshopper, Schistocerca americana, whose body size is not affected by rearing in oxygen levels as low as 5 kPa (Harrison et al. 2006). Even small insects such as D. melanogaster can be limited by relatively mild hypoxia, with the size of these flies decreasing linearly below 15 kPa aPO2 (Peck & Maddrell 2005). Hypoxia extends development times and decreases mean and maximal size, growth rates and survival (Loudon 1988; Greenberg & Ar 1996; Frazier et al. 2001; Klok et al. 2009). Hypoxic rearing reduces both cell size and number in Drosophila wings (Peck & Maddrell 2005). Similar developmental effects of oxygen on body size have been found in a wide variety of animals including humans (Bailey et al. 2007), alligators (Owerkowicz et al. 2009) and crustaceans (Seidl et al. 2005).

The morphology of the respiratory system of animals often compensates for changes in aPO2 (Sollid et al. 2003; Owerkowicz et al. 2009). Insects compensate for hypoxia by increasing tracheal diameters and the number of tracheoles (Jarecki et al. 1999; Henry & Harrison 2004). Many other compensatory changes occur in response to hypoxia, including down-regulation of metabolic enzymes (Zhou et al. 2007, 2008). Together, these physiological and developmental responses probably reduce negative effects of hypoxia on fitness.

(ii) Responses to hyperoxia

The effects of hyperoxia on growth and body size are less consistent and often nonlinear (Harrison et al. 2009). Body size increases in the giant mealworm, Z. morio (27% O2; Harrison et al. 2009) and in the scarabaeid beetle C. texana (40% O2; Harrison et al. 2009). However, in five tested species, there is no increase in body size at any tested level of hyperoxia, and M. sexta are smaller in hyperoxia (Harrison et al. 2009). Drosophila melanogaster reared in population bottles are larger when reared in hyperoxia (Frazier et al. 2001), but neither mean nor maximal sizes are larger when flies are reared individually, suggesting that hyperoxic effects on size in the population bottles are owing to evolutionary changes occurring in a single generation (Klok et al. 2009). The species showing the most positive responses of body size to hyperoxia are ground-dwelling beetles like Z. morio, suggesting that species which develop in occasionally hypoxic environments like soils may be more likely to benefit from atmospheric hyperoxia. In Z. morio, there is a distinct ∩-shaped response to hyperoxia, with mild hyperoxia increasing size and more extreme hyperoxia reducing size. The nonlinear and diverse developmental responses to hyperoxia in insects suggest that negative effects associated with oxidative damage overlap with stimulatory effects of higher aPO2. When hyperoxia increases body size, it does so primarily by extending development time rather than increasing growth rate (Greenberg & Ar 1996). Hyperoxic rearing leads to decreasing tracheal diameters and a reduced number of tracheoles (Jarecki et al. 1999; Henry & Harrison 2004). The effects of chronic hyperoxia have received little attention in vertebrates, but recent studies suggest that hyperoxia can increase the size and growth of embryonic and juvenile alligators, and reduce investment in cardiorespiratory structures (Owerkowicz et al. 2009).

(d) Evolutionary responses to aPO2

(i) Laboratory selection studies

Rearing D. melanogaster for multiple generations in hyperoxia results in evolution of increased mean and maximal body mass (Klok et al. 2009). The heaviest flies are obtained when flies reared for multiple generations in 40 kPa PO2 are returned to normoxia, suggesting involvement of parental effects. Rearing for multiple generations in hypoxia (either in 10 kPa PO2 or with stepwise decreasing aPO2 down to 4 kPa) causes flies to be smaller, but this effect is completely owing to developmental plasticity, as these flies are the same size as control flies when returned to normoxia (Zhou et al. 2007; Klok et al. 2009). Drosophila melanogaster may commonly encounter hypoxia in its natural habitat, which may explain why it has well-developed plastic responses to hypoxia. By contrast, hyperoxia is probably a novel situation for this species, and thus may be more likely to elicit evolutionary responses. The mechanisms that elicit evolution of larger size in D. melanogaster in response to hyperoxia remain unclear. Since survival is not affected (Frazier et al. 2001) and both mean and maximal sizes increase (Klok et al. 2009), it is plausible that larger flies gain fecundity advantages at higher oxygen levels.

Evolution of large size in the field is probably driven by natural or sexual selection. Does aPO2 interact with such selection, potentially synergistically, to affect evolution of size? At least in D. melanogaster, the answer appears to be ‘no’. Populations selected for large size do not differ in mean or maximal size when reared for multiple generations in 21 or 40 kPa PO2 (Klok & Harrison 2009). However, hypoxia (10 kPa PO2) strongly limited size even when flies are selected for large size (Klok & Harrison 2009). This effect is owing to plasticity, as these flies selected for large size in 10 kPa PO2 also have identical sizes as control flies when returned to normoxic conditions.

Lastly, D. melanogaster evolve larger tracheae when reared for multiple generations in hypoxia, and smaller tracheae when reared in hyperoxia (Henry & Harrison 2004), providing evidence for selection for such a compensatory strategy. This result suggests that tracheal investment has significant costs (materials, energy or space) that result in selection against excess tracheal structure.

(ii) Comparative studies

Habitat PO2 and insect body size

In a variety of aquatic invertebrates, habitat PO2 correlates positively with maximal and average body sizes (Peck & Chapelle 2003; Chapelle & Peck 2004), suggesting that oxygen availability has a strong effect on size in these groups. Terrestrial insects do not appear to exhibit consistent trends in body size across altitude-related aPO2, but changes in altitude are accompanied by potentially confounding changes in temperature, air density and growing season length (Dillon et al. 2006). Unfortunately, there is little data on how other natural hypoxic environments (i.e. organic soils, burrows) influence insect body size.

The relationship between species body size and critical PO2

Theoretically, oxygen delivery could be more challenging in larger species of insects owing to their longer tracheae, which could limit maximal insect size, providing a simple mechanism for oxygen effects on size evolution. However, empirical data indicate no effect of size on the critical PO2 values for oxygen delivery across resting grasshopper (Greenlee et al. 2007) or beetle species (Lease 2008). These and comparative data in other animal groups, like fishes (Nilsson & Östlund-Nilsson 2008), mammals (Weibel et al. 1998) and sea spiders (Woods et al. 2008) suggest that oxygen supply is morphologically and physiologically adjusted to demand across species body sizes. Large size might only be a problem for oxygen delivery during periods of higher oxygen consumption rates, such as running or flying at high temperatures. In support of this hypothesis, performance of larger bivalves becomes oxygen-limited at elevated temperatures that stimulate metabolism (Peck 2007). Perhaps, critical constraining effects of oxygen on large size occurs only during extreme environmental or physiological events.

Temperature and body size

Global temperatures were low during the late Carboniferous when some insects were gigantic (Royer et al. 2004), and interactions between temperature and oxygen could be part of the historical oxygen-size linkages. Lower temperatures tend to cause insects to be larger, via both direct developmental effects and by evolutionary changes in mean size (Kingsolver & Huey 2008; Chown & Gaston 2010). Rising temperatures exacerbate oxygen delivery problems and size responses to oxygen owing to exponential effects on metabolic rates (Frazier et al. 2001; Woods & Hill 2004). Lower temperatures could shorten the growing season, favouring evolution of larger insects more resistant to starvation (Chown & Gaston 2010). Lower temperatures and higher aPO2 could have expanded the available habitat for insects (Huey & Ward 2005); and since larger insects require a larger geographical range, this could have favoured larger species (Chown & Gaston 2010).

Tracheal investment and insect body size

Larger insects have lower mass-specific metabolic and gas exchange rates (Chown et al. 2007). Thus, we would predict that the tracheal system should scale hypometrically, as observed for mammalian capillary density and fish gills, or perhaps isometrically as observed for mammalian lungs (Weibel et al. 1998). However, the three studies that have investigated the scaling relationship of the tracheal system to date suggest that tracheal investment is hypermetric, with greater proportional investment in larger insects. During ontogeny of S. americana, tracheal investment increases in the leg muscle (Hartung et al. 2004) and at the whole-body level, with tracheal volumes and ventilation scaling approximately with mass1.3 (Lease et al. 2006; Greenlee et al. 2009). Similarly, across four tenebrionid beetle species, tracheal volumes scale with mass1.29 (Kaiser et al. 2007). Such a trend appears to be general for insects: tiny stick insects have tracheal volumes of around 2 per cent (Schmitz & Perry 1999), while giant scarabaeid beetles have tremendous air sacs (Miller 1966). Theoretical calculations suggest that the observed hypermetry is consistent with a need to overcome reduced rates of diffusive gas exchange in longer, blind-ended tracheoles (Harrison et al. 2009). Trends in compensation by differential increase of area-dependent structures cannot be continued indefinitely without producing structural absurdities.(Gould 1966)Increasing investment in a structure with size imposes constraints on larger animals. For example, larger vertebrates must have proportionally larger and thicker skeletons; this leads to more upright postures and reduced agility (Biewener 1989). Similarly, tracheal hypermetry may constrain insects. Tracheal hypermetry can potentially have multiple consequences on insect morphology and physiology that could result in selection against large insects including decreased density, increased cost of the respiratory system, displacement of other tissues and exhaustion of internal space available for tracheae.

Increasing animal volume per unit mass could affect many aspects of performance, e.g. increasing drag, lever arms for locomotion, or niche space required. Based on the scaling of the tracheal system in grasshoppers, a 1 kg grasshopper would have a volume of 3.7 l, greatly increasing the mass-specific need for nutrient investment in the exoskeleton and tracheal system, and probably the susceptibility to breakage (Greenlee et al. 2009). Hypermetry of the tracheal system could also lead to displacement of other tissues, reducing the performance of animals relative to those with a similar mass. The per cent of body volume occupied by non-respiratory tissues falls quadratically with mass, from 92 per cent in 10 mg grasshoppers to 59 per cent in 10 g animals, and extrapolated to 27 per cent in theoretical 1 kg grasshoppers (Greenlee et al. 2009). Potentially, survival or reproduction of larger grasshoppers could decrease relative to smaller individuals owing to reduced locomotory, digestive or reproductive capacities associated with such volume-specific decreases in functional tissue content.

Increasing tracheal hypermetry could also directly limit maximal insect size by filling all available space within key regions of the body that cannot be expanded for biomechanical reasons. In interspecific comparisons of beetles, the most dramatic example of hypermetry occurred at the connection between legs and body (Kaiser et al. 2007). Across four species of beetles, the cross-sectional area of the leg orifice scales with mass0.77, but the tracheal diameter penetrating the orifice scales with mass1.02 (above the 0.67 predicted by isometry), occupying increasingly larger fractions of the exoskeleton in larger animals. Extrapolating these trends of tracheal hypermetry indicates that in the largest extant beetle, the leg will be already 90 per cent full of trachea, suggesting a spatial limitation (Kaiser et al. 2007). Studies of the scaling of respiratory and non-respiratory structures in the largest extant insects are needed to test these extrapolations.

Because insect tracheal diameters are smaller and tracheoles are fewer when animals are reared at higher oxygen levels (Harrison et al. 2006), a higher aPO2 would allow insects to achieve larger size with smaller proportional tracheal volumes, with less material or spatial or energy cost of the respiratory system, with less displacement of other tissues, and before reaching spatial constraints. It is plausible that a reduced investment in the tracheal system at higher aPO2 is a benefit for insects of all sizes, but because insects experience tracheal hypermetry, it seems to be particularly important for larger insects, and thus a reasonable mechanism by which aPO2 might drive evolution of larger insect sizes. Further studies on the impact of oxygen on tracheal systems in a wider range of taxonomic groups and sizes of insects are necessary to support this hypothesis.

4. Alternative explanations for gigantism in the late palaeozoic and the small size of insects relative to vertebrates

Multiple alternative explanations are possible for Palaeozoic gigantism in insects; however, in general these have not been tested empirically. One possible explanation for Palaeozoic insect gigantism is niche displacement. Since the body sizes of animals respond to predation and competition (Blackburn & Gaston 1994), it is plausible that giant insects evolved into open niches, but that these species were later displaced by vertebrates filling the same ecological roles. Evolution of these giants has also been hypothesized to have occurred as a response to stable, optimal environmental conditions, and the availability of coal-swamp forests as a new habitat with little competition or predation (Briggs 1985). Such improved environmental conditions could directly increase insect abundances and/or shift body size distributions. The large size of some of the Palaeozoic insects could have been the result of size-selection by predators, or the need for greater force generation in ground litter filled with tree branches (Shear & Kukalova-Peck 1990).

Although the distribution of sizes of insects and vertebrates overlap, the mean and maximal sizes of terrestrial insects are smaller than for terrestrial vertebrates. As noted above, for many years it has been hypothesized that the small size of insects is partially owing to their use of a tracheal respiratory system; evidence that atmospheric hyperoxia might enable larger insects would substantially strengthen this argument. However, the observation that terrestrial crustaceans and arachnids lacking tracheae are also small relative to vertebrates suggests that other constraints may exist. Two possible constraints are the open circulatory system and the exoskeleton of arthropods.

In terrestrial species, blood must be pumped against gravity, suggesting that larger species would require higher pressures or reduced peripheral resistance. The open circulatory systems of terrestrial arthropods generate low pressures relative to the closed circulatory systems of vertebrates (Burggren et al. 1990; Ichikawa 2009). Such open circulatory systems might be unable to produce sufficient pressures to drive adequate flows in arthropods comparable in size to large vertebrates. Insects, however, possess accessory pulsatile organs that enhance blood flow in critical anatomical locations like legs, wings and antennae (Pass 2000), so it is possible that the possession of those accessory hearts renders high blood pressure unnecessary. Unfortunately, no study has yet tested the scaling of cardiovascular pressures or resistances in terrestrial arthropods for such limitations.

If the exoskeleton of larger arthropods should be as strong and durable as smaller animals, a greater proportion of their body needs to be dedicated to exoskeletal material, if the material properties do not change (Price 1997). For similar reasons, larger vertebrates have relatively thicker bones (McMahon 1973). Biomechanical constraints imposed by the exoskeleton on large insects may be particularly important during moulting, when the force of gravity on soft exoskeletons has the potential to ‘produce an animal like a great tough pancake’ (Currey 1970). The larger mass of aquatic arthropods (e.g. lobsters) compared with terrestrial ones supports a biomechanical constraint hypothesis, since aquatic organisms do not have to support their mass and thus experience less stress on their exoskeleton, especially during moulting (Smock 1980; Sabo et al. 2002). Exoskeletal thickness and energy storage of the leg does increase with size during ontogeny in locusts (Katz & Gosline 1992), but the mass of the exoskeleton scales isometrically with body mass in interspecific comparisons across insects (Lease 2008). As yet there are no data indicating that larger insects are more susceptible to exoskeletal damage, and as larger insect species do not invest more in the exoskeleton (Lease 2008), empirical support for a biomechanical constraint on size is lacking.

5. Conclusions

Insects larger than humans populate our movies and nightmares, but it seems unlikely that they will ever actually exist on earth. However, insects as large as sea gulls did exist in the Palaeozoic, and they stimulate our imagination. The complex interactions between aPO2, organisms and communities (figure 2) make it challenging to reject or accept the hypothesis that extant insects are small owing to possession of a tracheal respiratory system, and that hyperoxia enabled their Palaeozoic gigantism. Nonetheless, recent empirical data provide a wealth of plausible mechanisms by which aPO2 may play a critical role in the evolution of insect size. Although alternate hypotheses exist for both Palaeozoic gigantism and the small size of insects relative to vertebrates, these have received minimal experimental investigation. Improved palaeontological data, and multi-generational selection experiments examining effects of oxygen on size in environments that require activity and flight may be particularly useful in addressing these questions. As we contemplate potential major future changes in the Earth's ecosystem, it is of concern that we do not yet understand the mechanisms for such fundamental and striking biological patterns. Answers will come from a renewed focus on animal systems physiology, in collaboration with evolutionary biologists, ecologists, palaeontologists and geologists.

Acknowledgements

The research was partially funded by NSF IBN 0419704 and EAR 0746352 to J.F.H. J. H. Fewell provided helpful comments on the manuscript.

Footnotes

    • Received January 7, 2010.
    • Accepted February 19, 2010.
    • © 2010 The Royal Society

    References

    1. ↵
      1. Allen C. R.,
      2. Garmestani A. F.,
      3. Havlicek T. D.,
      4. Marquet P. A.,
      5. Peterson P. D.,
      6. Restrepo C.,
      7. Stow C. A.,
      8. Weeks B. E.
      2006 Patterns in body size distributions: sifting among alternative hypotheses. Ecol. Lett. 9, 630–648. (doi:10.1111/j.1461-0248.2006.00902.x)
      OpenUrlCrossRefPubMedWeb of Science
    2. ↵
      1. Bailey S. M.,
      2. Xu J.,
      3. Feng J. H.,
      4. Hu X.,
      5. Zhang C.,
      6. Qui S.
      2007 Tradeoffs between oxygen and energy in tibial growth at high altitude. Am. J. Hum. Biol. 19, 662–668. (doi:10.1002/ajhb.20667)
      OpenUrlCrossRefPubMedWeb of Science
    3. ↵
      1. Beckemeyer R. J.,
      2. Hall J. D.
      2007 The entomofauna of the lower Permian fossil insect beds of Kansas and Oklahoma, USA. Afr. Invertebrates 48, 23–39.
      OpenUrl
    4. ↵
      1. Bergman N. M.,
      2. Lenton T. M.,
      3. Watson A. J.
      2004 COPSE: a new model of biogeochemical cycling over Phanaerozoic time. Am. J. Sci. 304, 397–437. (doi:10.2475/ajs.304.5.397)
      OpenUrlAbstract/FREE Full Text
    5. ↵
      1. Berner R. A.
      2005 The carbon and sulfur cycles and atmospheric oxygen from middle Permian to middle Triassic. Geochim. Cosmochim. Acta 69, 3211–3217. (doi:10.1016/j.gca.2005.03.021)
      OpenUrlCrossRefGeoRefWeb of Science
    6. ↵
      1. Berner R. A.
      2006 a Carbon, sulfur and O2 across the Permian–Triassic boundary. J. Geochem. Exploration 88, 416–418. (doi:10.1016/j.gexplo.2005.08.088)
      OpenUrlCrossRef
    7. ↵
      1. Berner R. A.
      2006 b GEOCARBSULF: a combined model for Phanerozoic atmospheric O2 and CO2. Geochim. Cosmochim. Acta 70, 5653–5664. (doi:10.1016/j.gca.2005.11.032)
      OpenUrlCrossRefGeoRefWeb of Science
    8. ↵
      1. Berner R. A.,
      2. Canfield D. E.
      1989 A new model for atmospheric oxygen over phanerozoic time. Am. J. Sci. 289, 333–361.
      OpenUrlAbstract/FREE Full Text
    9. ↵
      1. Berner R. A.,
      2. VandenBrooks J. M.,
      3. Ward P. D.
      2007 Oxygen and evolution. Science 316, 557–558. (doi:10.1126/science.1140273)
      OpenUrlAbstract/FREE Full Text
    10. ↵
      1. Biewener A. A.
      1989 Scaling body support in mammals: limb posture and muscle mechanics. Science 245, 45–48. (doi:10.1126/science.2740914)
      OpenUrlAbstract/FREE Full Text
    11. ↵
      1. Blackburn T. M.,
      2. Gaston K. J.
      1994 Animal body size distributions: patterns, mechanisms and implications. Trends Ecol. Evol. 9, 471–474. (doi:10.1016/0169-5347(94)90311-5)
      OpenUrlCrossRefPubMedWeb of Science
    12. ↵
      1. Blanckenhorn W. U.
      2000 The evolution of body size: what keeps organisms small? Q. Rev. Biol. 75, 385–407. (doi:10.1086/393620)
      OpenUrlCrossRefPubMedWeb of Science
    13. ↵
      1. Briggs D. E. G.
      1985 Gigantism in palaeozoic arthropods. Special Pap. Palaeontol. 33, 1571.
      OpenUrl
    14. ↵
      1. Brown J. H.,
      2. Mauer B. A.
      1986 Body size, ecological dominance and Cope's rule. Nat. Austral. 324, 248–250. (doi:10.1038/324248a0)
      OpenUrl
    15. ↵
      1. Burggren W. W.,
      2. Pinder A. W.,
      3. McMahon B. R.,
      4. Doyle M.,
      5. Wheatly M.
      1990 Heart rate and hemolymph pressure responses to hemolymph volume changes in the land crab Cardisoma guanhumi: evidence for baroreflex regulation. Physiol. Zool. 63, 167–181.
      OpenUrl
    16. ↵
      1. Butterfield N. J.
      2009 Oxygen, animals and oceanic ventilation: an alternative view. Geobiology 7, 1–7. (doi:10.1111/j.1472-4669.2009.00188.x)
      OpenUrlCrossRefGeoRefPubMedWeb of Science
    17. ↵
      1. Carpenter F. M.
      1992 Treatise on invertebrate paleontology. Part R, Arthropoda 4, volumes 3 and 4 (Hexapoda). Lawrence, KS: University of Kansas Press.
    18. ↵
      1. Chapelle G.,
      2. Peck L. S.
      2004 Amphipod crustacean size spectra: new insights in the relationship between size and oxygen. Oikos 106, 167–175. (doi:10.1111/j.0030-1299.2004.12934.x)
      OpenUrlCrossRefWeb of Science
    19. ↵
      1. Chown S. L.,
      2. Gaston K. J.
      2010 Body size variation in insects: a macroecological perspective. Biol. Rev. 85, 139–169. (doi:10.1111/j.1469-185X.2009.00097.x)
      OpenUrlCrossRefPubMed
    20. ↵
      1. Chown S. L.,
      2. Marais E.,
      3. Terblanche J. S.,
      4. Klok C. J.,
      5. Lighton J. R. B.,
      6. Blackburn T. M.
      2007 Scaling of insect metabolic rate is inconsistent with the nutrient supply network model. Funct. Ecol. 21, 282–290. (doi:10.1111/j.1365-2435.2007.01245.x)
      OpenUrlCrossRefWeb of Science
    21. ↵
      1. Currey J. D.
      1970 Animal skeletons. London, UK: Edward Arnold.
    22. ↵
      1. Dekanty A.,
      2. Centanin L.,
      3. Wappner P.
      2007 Role of the hypoxia-response pathway on cell size determination and growth control. Dev. Biol. 306, 339–339. (doi:10.1016/j.ydbio.2007.03.182)
      OpenUrl
    23. ↵
      1. Dillon M. E.,
      2. Frazier M. R.,
      3. Dudley R.
      2006 Into thin air: physiology and evolution of alpine insects. Integr. Comp. Biol. 46, 49–61. (doi:10.1093/icb/icj007)
      OpenUrlAbstract/FREE Full Text
    24. ↵
      1. Dudley R.
      1998 Atmospheric oxygen, giant Paleozoic insects and the evolution of aerial locomotor performance. J. Exp. Biol. 201, 1043–1050.
      OpenUrlAbstract
    25. ↵
      1. Dudley R.
      2000 The evolutionary physiology of animal flight: paleobiological and present perspectives. Annu. Rev. Physiol. 62, 135–155. (doi:10.1146/annurev.physiol.62.1.135)
      OpenUrlCrossRefPubMedWeb of Science
    26. ↵
      1. Falkowski P. G.,
      2. Katz M. E.,
      3. Milligan A. J.,
      4. Fennel K.,
      5. Cramer B. S.,
      6. Aubry M. P.,
      7. Berner R. A.,
      8. Novacek M. J.,
      9. Zapol W. M.
      2005 The rise of oxygen over the past 205 million years and the evolution of large placental mammals. Science 309, 2202–2204. (doi:10.1126/science.1116047)
      OpenUrlAbstract/FREE Full Text
    27. ↵
      1. Frazier M. R.
      2007 Alpine insects: physiology and evolution in cold, thin air. PhD thesis, University of Washington, Seattle.
    28. ↵
      1. Frazier M. R.,
      2. Woods H. A.,
      3. Harrison J.
      2001 Interactive effects of rearing temperature and oxygen on the development of Drosophila melanogaster. Physiol. Biochem. Zool. 74, 641–650. (doi:10.1086/322172)
      OpenUrlCrossRefPubMedWeb of Science
    29. ↵
      1. Gorr T. A.,
      2. Gassmann M.,
      3. Wappner P.
      2006 Sensing and responding to hypoxia via HIF in model invertebrates. J. Insect Physiol. 52, 349–364. (doi:10.1016/j.jinsphys.2006.01.002)
      OpenUrlCrossRefPubMedWeb of Science
    30. ↵
      1. Gould S. J.
      1966 Allometry and size in ontogeny and phylogeny. Biol. Rev. 41, 587–640. (doi:10.1111/j.1469-185X.1966.tb01624.x)
      OpenUrlCrossRefGeoRefWeb of Science
    31. ↵
      1. Graham J. B.,
      2. Dudley R.,
      3. Aguilar N. M.,
      4. Gans C.
      1995 Implications of the later Palaeozoic oxygen pulse for physiology and evolution. Nature 375, 117–120. (doi:10.1038/375117a0)
      OpenUrlCrossRefGeoRef
    32. ↵
      1. Greenberg S.,
      2. Ar A.
      1996 Effects of chronic hypoxia, normoxia and hyperoxia on larval development in the beetle Tenebrio molitor. J. Insect Physiol. 42, 991–996. (doi:10.1016/S0022-1910(96)00071-6)
      OpenUrlCrossRefWeb of Science
    33. ↵
      1. Greenlee K. J.,
      2. Harrison J. F.
      2004 a Development of respiratory function in the American locust Schistocerca americana. I. Across-instar effects. J. Exp. Biol. 207, 497–508. (doi:10.1242/jeb.00767)
      OpenUrlAbstract/FREE Full Text
    34. ↵
      1. Greenlee K. J.,
      2. Harrison J. F.
      2004 b Development of respiratory function in the American locust Schistocerca americana. II. Within-instar effects. J. Exp. Biol. 207, 509–517. (doi:10.1242/jeb.00766)
      OpenUrlAbstract/FREE Full Text
    35. ↵
      1. Greenlee K. J.,
      2. Harrison J. F.
      2005 Respiratory changes throughout ontogeny in the tobacco hornworm caterpillar, Manduca sexta. J. Exp. Biol. 208, 1385–1392. (doi:10.1242/jeb.01521)
      OpenUrlAbstract/FREE Full Text
    36. ↵
      1. Greenlee K. J.,
      2. Nebeker C.,
      3. Harrison J. F.
      2007 Body size-independent safety margins for gas exchange across grasshopper species. J. Exp. Biol. 210, 1288–1296. (doi:10.1242/jeb.001982)
      OpenUrlAbstract/FREE Full Text
    37. ↵
      1. Greenlee K. J.,
      2. Henry J. R.,
      3. Kirkton S. D.,
      4. Westneat M. W.,
      5. Fezzaa K.,
      6. Lee W. K.,
      7. Harrison J. F.
      2009 Synchrotron imaging of the grasshopper tracheal system: morphological components of tracheal hypermetry and the effect of age and stage on abdominal air sac volumes and convection. Am. J. Physiol. Regul. Integr. Comp. Physiol. 297, 1343–1350. (doi:10.1242/jeb.00767)
      OpenUrl
    38. ↵
      1. Grimaldi D.,
      2. Engel M. S.
      2005 Evolution of the insects. New York, NY: Cambridge University Press.
    39. ↵
      1. Haldane J. B. S.
      1926 On being the right size. Harper's Mon. Mag. 152, 424–427.
      OpenUrl
    40. ↵
      1. Harrison J. F.,
      2. Lighton J. R. B.
      1998 Oxygen-sensitive flight metabolism in the dragonfly Erythemis simplicicollis. J. Exp. Biol. 201, 1739–1744.
      OpenUrlAbstract
    41. ↵
      1. Harrison J.,
      2. Frazier M. R.,
      3. Henry J. R.,
      4. Kaiser A.,
      5. Klok C. J.,
      6. Rascon B.
      2006 Responses of terrestrial insects to hypoxia or hyperoxia. Resp. Physiol. Neurobiol. 154, 4–17. (doi:10.1016/j.resp.2006.02.008)
      OpenUrlCrossRef
    42. ↵
      1. Morris S.,
      2. Vosloo A.
      1. Harrison J. F.,
      2. Kaiser A.,
      3. VandenBrooks J. M.
      2009 Mysteries of oxygen and insect size. In 4th CPB Meeting in Africa: Mara 2008. Molecules to migration: the pressures of life (eds Morris S., Vosloo A.), pp. 293–302. Bologna, Italy: Medimond Publishing.
    43. ↵
      1. Hartung D. K.,
      2. Kirkton S. D.,
      3. Harrison J. F.
      2004 Ontogeny of tracheal system structure: a light and electron-microscopy study of the metathoracic femur of the American locust, Schistocerca americana. J. Morphol. 262, 800–812. (doi:10.1002/jmor.10281)
      OpenUrlCrossRefPubMedWeb of Science
    44. ↵
      1. Henry J. R.,
      2. Harrison J. F.
      2004 Plastic and evolved responses of larval tracheae and mass to varying atmospheric oxygen content in Drosophila melanogaster. J. Exp. Biol. 207, 3559–3567. (doi:10.1242/jeb.01189)
      OpenUrlAbstract/FREE Full Text
    45. ↵
      1. Huey R. B.,
      2. Ward P. D.
      2005 Hypoxia, global warming, and terrestrial late Permian extinctions. Science 308, 398–401. (doi:10.1126/science.1108019)
      OpenUrlAbstract/FREE Full Text
    46. ↵
      1. Ichikawa T.
      2009 Mechanism of hemolymph circulation in the pupal leg of tenebrionid beetle Zophobas atratus. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 153, 174–180. (doi:10.1016/j.cbpa.2009.02.006)
      OpenUrlCrossRef
    47. ↵
      1. Jarecki J.,
      2. Johnson E.,
      3. Krasnow M. A.
      1999 Oxygen regulation of airway branching in Drosophila is mediated by branchless FGF. Cell 99, 211–220. (doi:10.1016/S0092-8674(00)81652-9)
      OpenUrlCrossRefPubMedWeb of Science
    48. ↵
      1. Jones J. H.
      1994 Comparative vertebrate exercise physiology: phyletic adaptations. San Diego, CA: Academic Press.
    49. ↵
      1. Joos B.,
      2. Lighton J. R. B.,
      3. Harrison J. F.,
      4. Suarez R. K.,
      5. Roberts S. P.
      1997 Effects of ambient oxygen tension on flight performance, metabolism, and water loss of the honeybee. Physiol. Zool. 70, 167–174.
      OpenUrlPubMed
    50. ↵
      1. Kaiser A.,
      2. Klok C. J.,
      3. Socha J. J.,
      4. Lee W.-K.,
      5. Quinlan M. C.,
      6. Harrison J. F.
      2007 Increase in tracheal investment with beetle size supports hypothesis of oxygen limitation on insect gigantism. Proc. Natl Acad. Sci. USA 104, 13 198–13 203. (doi:10.1073/pnas.0611544104)
      OpenUrlAbstract/FREE Full Text
    51. ↵
      1. Katz S. L.,
      2. Gosline J. M.
      1992 Ontogenentic scaling and mechanical behaviour of the tibiae of the African desert locust (Schistocerca gregaria). J. Exp. Biol. 168, 125–150.
      OpenUrlAbstract/FREE Full Text
    52. ↵
      1. Kingsolver J.,
      2. Huey R. B.
      2008 Size, temperature and fitness. Three rules. Evol. Ecol. Res. 10, 251–268.
      OpenUrl
    53. ↵
      1. Kirkton S. D.,
      2. Niska J. A.,
      3. Harrison J. F.
      2005 Ontogenetic effects on aerobic and anaerobic metabolism during jumping in the American locust, Schistocerca americana. J. Exp. Biol. 208, 3003–3012. (doi:10.1242/jeb.01747)
      OpenUrlAbstract/FREE Full Text
    54. ↵
      1. Klok C. J.,
      2. Harrison J. F.
      2009 Atmospheric hypoxia limits selection for large body size in insects. PLoS ONE 4, e3876. (doi:10.1371/journal.pone.0003876)
      OpenUrlCrossRefPubMed
    55. ↵
      1. Klok C. J.,
      2. Hubb A. J.,
      3. Harrison J. F.
      2009 Single and multigenerational responses of body mass to atmospheric oxygen concentrations in Drosophila melanogaster: evidence for roles of plasticity and evolution. J. Evol. Biol. 22, 2496–2504. (doi:10.1111/j.1420-9101.2009.01866.x)
      OpenUrlCrossRefPubMedWeb of Science
    56. ↵
      1. Kukalova-Peck J.
      1985 Ephemeroid wing venation based upon new gigantic Carboniferous mayflies and basic morphology, phylogeny, and metamorphosis of pterygote insects (Insecta, Ephemerida). Can. J. Zool. 63, 933–955. (doi:10.1139/z85-139)
      OpenUrlCrossRef
    57. ↵
      1. Labandera C. C.,
      2. Sepkoski J. J.
      1993 Insect diversity in the fossil record. Science 261, 310–315. (doi:10.1126/science.11536548)
      OpenUrlAbstract/FREE Full Text
    58. ↵
      1. Lease H. M.
      2008 Does size matter? The scaling of respiration, energy reserves, and support structures in arthropods. PhD thesis, University of New Mexico, Albuquerque.
    59. ↵
      1. Lease H. M.,
      2. Wolf B. O.,
      3. Harrison J. F.
      2006 Intraspecific variation in tracheal volume in the American locust, Schistocerca americana, measured by a new inert gas method. J. Exp. Biol. 209, 3476–3483. (doi:10.1242/jeb.02343)
      OpenUrlAbstract/FREE Full Text
    60. ↵
      1. Lighton J. R. B.,
      2. Schilman P. E.,
      3. Holway D. A.
      2004 The hyperoxic switch: assessing respiratory water loss rates in tracheate arthropods with continuous gas exchange. J. Exp. Biol. 207, 4463–4471. (doi:10.1242/jeb.01284)
      OpenUrlAbstract/FREE Full Text
    61. ↵
      1. Loudon C.
      1988 Development of Tenebrio molitor in low oxygen levels. J. Insect Physiol. 34, 97–103. (doi:10.1016/0022-1910(88)90160-6)
      OpenUrlCrossRefWeb of Science
    62. ↵
      1. May M. L.
      1982 Heat exchange and endothermy in protodonata. Evolution 36, 1051–1058. (doi:10.2307/2408082)
      OpenUrlCrossRefWeb of Science
    63. ↵
      1. McAlester A. L.
      1970 Animal extinctions, oxygen consumption, and atmospheric history. J. Paleontol. 44, 405–409.
      OpenUrlAbstract
    64. ↵
      1. McMahon T.
      1973 Size and shape in biology; elastic criteria impose limits on biological proportions, and consequently on metabolic rates. Science 179, 1201–1203. (doi:10.1126/science.179.4079.1201)
      OpenUrlAbstract/FREE Full Text
    65. ↵
      1. Miller P. L.
      1966 The supply of oxygen to the active flight muscles of some large beetles. J. Exp. Biol. 45, 285–304.
      OpenUrlAbstract/FREE Full Text
    66. ↵
      1. Mirth K. M.,
      2. Riddiford L. M.
      2007 Size assessment and growth control: how adult size is determined in insects. Bioessays 29, 344–355. (doi:10.1002/bies.20552)
      OpenUrlCrossRefPubMedWeb of Science
    67. ↵
      1. Nijhout H. F.
      2006 A quantitative analysis of the mechanism that controls the body size in Manduca sexta. J. Biol. 5. (doi:10.1186/jbiol43)
    68. ↵
      1. Nilsson G. E.,
      2. Östlund-Nilsson S.
      2008 Does size matter for hypoxia tolerance in fish? Biol. Rev. 83, 173–189. (doi:10.1111/j.1469-185X.2008.00038.x)
      OpenUrlCrossRefPubMed
    69. ↵
      1. Owerkowicz T.,
      2. Elsey R. M.,
      3. Hicks J. W.
      2009 Atmospheric oxygen level affects growth trajectory, cardiopulmonary allometry and metabolic rate in the American alligator (Alligator mississippiensis). J. Exp. Biol. 212, 1237–1247. (doi:10.1242/jeb.023945)
      OpenUrlAbstract/FREE Full Text
    70. ↵
      1. Pass G.
      2000 Accessory pulsatile organs: evolutionary innovations in insects. Annu. Rev. Entomol. 45, 495–518. (doi:10.1146/annurev.ento.45.1.495)
      OpenUrlCrossRefPubMedWeb of Science
    71. ↵
      1. Peck L.
      2007 Oxygen limited thermal tolerance: the scale of individual variation, and the effects of size. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 146, S207–S207. (doi:10.1016/j.cbpa.2007.01.460)
      OpenUrl
    72. ↵
      1. Peck L. S.,
      2. Chapelle G.
      2003 Reduced oxygen at high altitude limits maximum size. Proc. R. Soc. Lond. B 270, S166–S167. (doi:10.1098/rsbl.2003.0054)
      OpenUrlAbstract/FREE Full Text
    73. ↵
      1. Peck L. S.,
      2. Maddrell S. H. P.
      2005 Limitation of size by hypoxia in the fruit fly Drosophila melanogaster. J. Exp. Zool. A Comp. Exp. Biol. 303A, 968–975. (doi:10.1002/jez.a.211)
      OpenUrlPubMed
    74. ↵
      1. Price P. W.
      1997 Insect ecology. New York, NY: Wiley.
    75. ↵
      1. Rascón B.,
      2. Harrison J. F.
      2005 Oxygen partial pressure effects on metabolic rate and behavior of tethered flying locusts. J. Insect Physiol. 51, 1193–1199. (doi:10.1016/j.jinsphys.2005.06.008)
      OpenUrlCrossRefPubMedWeb of Science
    76. ↵
      1. Royer D. L.,
      2. Berner R. A.,
      3. Montanez I. P.,
      4. Tabor N. J.
      2004 CO2 as a primary driver for Phanerozoic climate. GSA Today 14, 4–10. (doi:10.1130/1052-5173(2004)014<4:CAAPDO>2.0.CO;2)
      OpenUrlCrossRefGeoRef
    77. ↵
      1. Sabo J. L.,
      2. Bastow J. L.,
      3. Power M. E.
      2002 Length-mass relationships for adult aquatic and terrestrial invertebrates in a California watershed. J. North Am. Benthol. Soc. 21, 336–343. (doi:10.2307/1468420)
      OpenUrlCrossRef
    78. ↵
      1. Schmitz A.,
      2. Perry S. F.
      1999 Stereological determination of tracheal volume and diffusing capacity of the tracheal walls in the stick insect Carausius morosus (Phasmatodea, Lonchodidae). Physiol. Biochem. Zool. 72, 205–218. (doi:10.1086/316655)
      OpenUrlCrossRefPubMedWeb of Science
    79. ↵
      1. Seidl M. D.,
      2. Paul R. J.,
      3. Pirow R.
      2005 Effects of hypoxia acclimation on morpho-physiological traits over three generations of Daphnia magna. J. Exp. Biol. 208, 2165–2175. (doi:10.1242/jeb.01614)
      OpenUrlAbstract/FREE Full Text
    80. ↵
      1. Shear W. A.,
      2. Kukalova-Peck J.
      1990 The ecology of Paleozoic terrestrial arthropods: the fossil evidence. Can. J. Zool. 68, 1807–1834. (doi:10.1139/z90-262)
      OpenUrlCrossRefWeb of Science
    81. ↵
      1. Smock L. A.
      1980 Relationships between body size and biomass of aquatic insects. Freshwater Biol. 10, 375–383. (doi:10.1111/j.1365-2427.1980.tb01211.x)
      OpenUrlCrossRef
    82. ↵
      1. Sollid J.,
      2. De Angelis P.,
      3. Gundersen K.,
      4. Nilsson G. E.
      2003 Hypoxia induces adaptive and reversible gross morphological changes in crucian carp gills. J. Exp. Biol. 206, 3667–3673. (doi:10.1242/jeb.00594)
      OpenUrlAbstract/FREE Full Text
    83. ↵
      1. VandenBrooks J. M.
      2007 The effects of varying partial pressure of oxygen on vertebrate development and evolution. PhD thesis, Yale University, New Haven, CT.
    84. ↵
      1. Ward P. D.
      2006 Out of thin air: dinosaurs, birds and Earth's ancient atmosphere. Washington, DC: Joseph Henry Press.
    85. ↵
      1. Weibel E. R.,
      2. Taylor C. R.,
      3. Bolis L.
      1998 Principles of animal design. The optimization and symmorphosis debate. Cambridge, UK: Cambridge University Press.
    86. ↵
      1. Winter A.,
      2. Ciborowski J. J. H.,
      3. Reynoldson T. B.
      1996 Effects of chronic hypoxia and reduced temperature on survival and growth of burrowing mayflies, Hexagenia limbata (Ephemeroptera: Ephemeridae). Can. J. Fish. Aquat. Sci. 53, 1565–1571. (doi:10.1139/cjfas-53-7-1565)
      OpenUrlCrossRef
    87. ↵
      1. Woods H. A.,
      2. Hill R. I.
      2004 Temperature-dependent oxygen limitation in insect eggs. J. Exp. Biol. 207, 2267–2276. (doi:10.1242/jeb.00991)
      OpenUrlAbstract/FREE Full Text
    88. ↵
      1. Woods H. A.,
      2. Moran A. L.,
      3. Arango C. P.,
      4. Mullen L.,
      5. Shields C.
      2008 Oxygen hypothesis of polar gigantism not supported by performance of Antarctic pycnogonids in hypoxia. Proc. R. Soc. B 276, 1069–1075. (doi:10.1098/rspb.2008.1489)
      OpenUrlCrossRefWeb of Science
    89. ↵
      1. Zhou D.,
      2. Xue J.,
      3. Chen J.,
      4. Morcillo P.,
      5. Lambert J. D.,
      6. White K. P.,
      7. Haddad G. G.
      2007 Experimental selection for Drosophila survival in extremely low O2 environment. PLoS ONE 2, e490. (doi:10.1371/journal.pone.0000490)
      OpenUrlCrossRefPubMed
    90. ↵
      1. Zhou D.,
      2. Xue J.,
      3. Lai J. C. K.,
      4. Schork N. J.,
      5. White K. P.,
      6. Haddad G. G.
      2008 Mechanisms underlying hypoxia tolerance in Drosophila melanogaster: hairy as a metabolic switch. PLoS Genet. 4, e1000221. (doi:10.1371/journal.pgen.1000221)
      OpenUrlCrossRefPubMed
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    7 July 2010
    Volume 277, issue 1690
    Proceedings of the Royal Society B: Biological Sciences: 277 (1690)
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    Atmospheric oxygen level and the evolution of insect body size
    Jon F. Harrison, Alexander Kaiser, John M. VandenBrooks
    Proc. R. Soc. B 2010 277 1937-1946; DOI: 10.1098/rspb.2010.0001. Published 26 May 2010
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    Atmospheric oxygen level and the evolution of insect body size

    Jon F. Harrison, Alexander Kaiser, John M. VandenBrooks
    Proc. R. Soc. B 2010 277 1937-1946; DOI: 10.1098/rspb.2010.0001. Published 26 May 2010

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    • Article
      • Abstract
      • 1. Introduction
      • 2. Geological and palaeontological evidence for changes in atmospheric oxygen level as a cause of historical insect gigantism
      • 3. Mechanisms for oxygen effects on insect body size
      • 4. Alternative explanations for gigantism in the late palaeozoic and the small size of insects relative to vertebrates
      • 5. Conclusions
      • Acknowledgements
      • Footnotes
      • References
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